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Immunological characterization of the endoproteases PC1 and PC2 in adrenal chromaffin granules and in the pituitary gland
Author(s) -
Kirchmair Rudolf,
Gee Patricia,
Hogue-Angeletti Ruth,
Laslop Andrea,
Fischer-Colbrie Reiner,
Winkler Hans
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80560-4
Subject(s) - antiserum , glycoprotein , anterior pituitary , pituitary gland , adrenal gland , adrenocorticotropic hormone , microbiology and biotechnology , chemistry , biochemistry , biology , endocrinology , immunology , antibody , hormone
Specific antisera against synthetic Fragments of the endoproteases, PC1 and PC2, were used to characterize these proteins. In one‐dimensional immunoblots these antisera labelled components of 85 kDa for PC1 and of 70 kDa for PC2 in purified bovine chromaffin granules and anterior and posterior pituitary of ox and rat. In membranes of bovine chromaffin granules glycoprotein H was identified as the major PC2 immunoreactive spot. A major part of these endoproteases appeared membrane bound.