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Hepatocyte growth factor (HGF) mediates the sustained formation of 1,2‐diacylglycerol via phosphatidylcholine—phospholipase C in cultured rat hepatocytes
Author(s) -
Osada Shinji,
Nakashima Shigeru,
Saji Shigetoyo,
Nakamura Toshikazu,
Nozawa Yoshinori
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80554-t
Subject(s) - phospholipase c , diacylglycerol kinase , hepatocyte growth factor , hepatocyte , phospholipase , phospholipase d , phosphatidylcholine , phosphoinositide phospholipase c , inositol , diglyceride , chemistry , gq alpha subunit , biology , microbiology and biotechnology , medicine , endocrinology , signal transduction , biochemistry , enzyme , protein kinase c , phospholipid , receptor , in vitro , g protein , membrane
The addition of hepatocyte growth factor (HGF) to rat hepatocytes in primary culture resulted in the formation of inositol 1,4,5‐trisphosphate (Ins(1,4,5)P 3 ) and 1,2‐diacylglycerol (DG) by a phosphoinositide‐specific phospholipase C (PI‐PLC). DG showed a biphasic increase; the first phase, corresponding with the peak of Ins(1,4,5)P 3 and a second larger and prolonged phase. The HGF stimulates the phosphatidylcholine (PC)‐derived prolonged DG formation by a phospholipase C pathway (PC‐PLC) but not by a phospholipase D pathway. HGF also was found to elicit [Ca 2+ ] oscillations which may be associated with the prolonged DG production from PC via the PC‐PLC phospholipase C pathway.