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Southern molecular hybridization experiments with parallel complementary DNA probes
Author(s) -
Tchurikov Nickolai A.,
Shchyolkina Anna K.,
Borissova Olga F.,
Chernov Boris K.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80545-r
Subject(s) - ethidium bromide , dna , southern blot , antiparallel (mathematics) , microbiology and biotechnology , nucleic acid thermodynamics , chemistry , hybridization probe , molecular probe , biology , biochemistry , base sequence , physics , quantum mechanics , magnetic field
We have detected the specific binding in Southern blot hybridization experiments of both complementary antiparallel and parallel 40 bp synthetic DNA probes, corresponding to a cloned Drosophila DNA fragment. The highly cooperative annealing and melting were observed in solution with these probes, which are complementary in the same direction and possess 17 GC pairs. The binding of ethidium bromide is indicative of formation of a perfect parallel DNA duplex. The specific binding was also detected in both genomic and in plaque hybridization experiments.