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Native‐type DHP‐sensitive calcium channel currents are produced by cloned rat aortic smooth muscle and cardiac α 1 subunits expressed in Xenopus laevis ooeytes and are regulated by α 2 ‐ and β‐subunits
Author(s) -
Itagaki Kiyoshi,
Koch Walter J.,
Bodi Ilona,
Klöckner Udo,
Slish Donald F.,
Schwartz Arnold
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80542-o
Subject(s) - xenopus , l type calcium channel , calcium , salientia , cardiac muscle , protein subunit , chemistry , voltage dependent calcium channel , anatomy , microbiology and biotechnology , calcium channel , smooth muscle , biophysics , biology , biochemistry , endocrinology , gene , organic chemistry
Native tissue‐like L‐type voltage‐dependent calcium channels (L‐VDCC's) were expressed by in vitro transcribed cRNA injection of rat aorta or rabbit cardiac α 1 subunit into Xenopus laevis oocytes. Co‐injection of VSM‐α 1 with the cloned skeletal muscle β‐subunit (SK‐β) of the L‐type VDCC significantly increased the expressed peak current amplitude without significant changes in kinetics. Similar results were obtained by co‐injection of cardiac α 1 (DSHT‐α 1 ) the cloned skeletal α 2 ‐subunit (SK‐α 2 ) or with SK‐β. The oocytes co‐expressing cRNA's retained L‐type VDCC pharmacology.