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Studies on the proteins involved in the interaction of high‐density lipoprotein with isolated human small intestine epithelial cells
Author(s) -
Sviridov Dd,
Christian Ehnholm,
H Tenkanen,
Pavlov MYu,
Safonova Ig,
V. S. Repin
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80519-m
Subject(s) - polyclonal antibodies , apolipoprotein b , antibody , lipoprotein , intracellular , biochemistry , microbiology and biotechnology , receptor , high density lipoprotein , chemistry , binding protein , biology , cholesterol , gene , immunology
Treatment of 125 I‐labelled high‐density lipoprotein ([ 125 I]HDL 3 ) with monospecific polyclonal antibodies against apolipoproteins A‐I and A‐II resulted in a dose‐dependent inhibition of the [ 125 I]HDL 3 binding to isolated human small intestine epithelial cells by 25%, and 50%, respectively. Both antibodies also inhibited intracellular degradation of [ 125 I]HDL 3 by 80%. Treatment of enterocytes with polyclonal antibody against apolipoprotein A‐I binding protein, a putative HDL receptor, inhibited both binding and degradation of [ 125 I]HDL 3 by these cells by 50%. Antibodies to apolipoprotein A‐I, A‐II and apo A‐I‐binding protein also inhibited [ 125 I]HDL 3 binding to cholesterol‐loaded cells.