Premium
Demonstration of DNA binding factors interacting with a fragment of the canine prostate arginine esterase gene promoter
Author(s) -
Pierre Chapdelaine,
Sylvain L. Guérin,
Tremblay Rr,
Dubé Jy
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80501-7
Subject(s) - arginine , gene , microbiology and biotechnology , dna , esterase , biology , androgen receptor , chemistry , biochemistry , enzyme , genetics , prostate cancer , amino acid , cancer
We have studied, by the gel mobility shift assay, the interaction of DNA binding proteins with a fragment of the proximal promoter (from nucleotides −177 to −47) of the androgen‐regulated canine prostate arginine esterase gene. Several shifted bands were obtained using nuclear extracts from various tissues. In the case of the prostate, the intensity of some of the shifted bands was decreased or increased when the extracts were prepared from animals that had been castrated 12 days earlier. Several of the DNA‐protein complexes could be assigned to an interaction with part or all of the sequence T from −124 to −114. We also obtained evidence for the presence of protein(s) interacting with an Spl motif present in the same fragment. These results suggest that some ubiquitous factors different from the androgen receptors could be involved in the regulation of the arginine esterase gene.