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Comment to Shinohara et al. (1991) FEBS Letters 293, 1731̄74 The uncoupling protein is not expressed in rat liver
Author(s) -
Ricquier Daniel,
Raimbault Serge,
Champigny Odette,
Miroux Bruno,
Bouillaud Frédéric
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80496-4
Subject(s) - complementary dna , western blot , messenger rna , uncoupling protein , biology , microbiology and biotechnology , gene expression , biochemistry , gene , brown adipose tissue , adipose tissue
Using Northern blot analysis, immunoblotting with purified antibodies and Polymerase Chain Reaction analysis, we were unable to detect the Uncoupling Protein‐UCP or its mRNA in liver of control, cold‐exposed or newborn rats. The unique expression of this protein in brown adipocytes was confirmed. These data refute the surprising recent report on UCP expression in rat liver (Shinohara (1991) FEBS Lett. 293, 173–174). Moreover we report that the hybridization signal obtained by these authors is probably non‐specific and due to the 3′ non‐coding domain of the UCP cDNA probe.