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IL‐1β, a strong mediator for glucose uptake by rheumatoid and non‐rheumatoid cultured human synoviocytes
Author(s) -
Hernvann A.,
Aussel C.,
Cynober L.,
Moatti N.,
Ekindjian O.G.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80481-u
Subject(s) - stimulation , endocrinology , medicine , basal (medicine) , chemistry , rheumatoid arthritis , prostaglandin e2 , synovial membrane , secretion , interleukin , glucose uptake , inflammation , cytokine , diabetes mellitus , insulin
Higher basal 2‐deoxy‐ d ‐glucose uptake in rheumatoid synovial cells than in non‐rheumatoid synovial cells, was found to be associated with an increased interleukin‐1β (IL‐1β) secretion (respectively 850 ± 238 vs. 8.3 ± 2.4 pg/24 h/10 5 cells, mean ± S.E.M.). When exogenous human recombinant IL‐1β was added to cultures, a marked stimulation of 2‐deoxy‐ d ‐glucose uptake was performed by both human synovial cultured cells, in a time‐dependent and dose‐dependent manner (IL‐1β0–100 ng/ml). In non‐rheumatoid synoviocytes, stimulation occurred 1–3 h following the addition of 1 ng/ml interleukin‐1β and increased up to 24 hours (respectively +150% and +261.4% after 6 and 24 hours association time). Rheumatoid synovial cells were less sensitive to 1 ng/ml IL‐1β (respectively +80% and +146.4%). IL‐1β increased significantly the V max for 2‐deoxy‐ d ‐glycose uptake by synovial cells, with no change in the K m . This effect was protein synthesis‐dependent, and not secondary to prostaglandin E2 synthesis or cell growth. IL‐1β possesses an important effect on glucose homeostasis in synovial cells, which could be indirect and/or regulated by the presence of natural inhibitors.