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Effects of 2(3)‐ tert ‐butyl‐4‐hydroxyanisole (BHA) on in situ mitochondria of Trypanosoma cruzi
Author(s) -
Aldunate J.,
Coloma-Torres L.,
Spencer P.,
Morello A.,
Ojeda J.M.,
Repetto Y.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80480-5
Subject(s) - trypanosoma cruzi , mitochondrion , rotenone , respiratory chain , biochemistry , electron transport chain , cytochrome c , eukaryote , mitochondrial respiratory chain , biology , oxidative phosphorylation , in situ , cytochrome , chemistry , stereochemistry , parasite hosting , enzyme , organic chemistry , genome , world wide web , computer science , gene
Results obtained with in situ mitochondria or Trypanosoma cruzi showed that this protozoon had only two energy coupling sites, sites II and III that correspond to higher eukaryote mitochondria. Rotenone did not inhibit the oxygen uptake or the parasite. These results suggest that the NADH‐ubiquinone segment of the respiratory chain has no activity. Studies with in situ mitochondria confirmed that BHA, an antioxidant food additive, blocks the mitochondrial electron transport chain at the succinate‐cytochrome b segment being the molecular basis of this trypanocidal action.