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H7, a protein kinase C inhibitor, increases the glutathione content of neuroblastoma cells
Author(s) -
Romero Francisco J.,
Llopis Juan,
Felipo Vicente,
Miñana Maria-Dolores,
Romá Joaquín,
Grisolía Santiago
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80468-v
Subject(s) - staurosporine , protein kinase c , glutathione , intracellular , sphingosine , kinase , chemistry , protein kinase a , neuroblastoma , protein kinase inhibitor , microbiology and biotechnology , biochemistry , biology , cell culture , enzyme , receptor , genetics
It is shown that the intracellular glutathione (GSH) concentration of neuroblastoma‐2a cells in culture increases with a maximum at 24 h after starting treatment with 1‐(5‐isoquinolinylsulfonyl)‐2‐methylpiperazine (H7), an inhibitor of protein kinase C (PKC). Other inhibitors of this and other protein kinases, e.g. sphingosine, staurosporine, and HA 1004, at the concentrations tested, had a less marked or negligible effect on intracellular GSH concentration. 12‐ O ‐Tetradecanoylphorbol‐13‐acetate (TPA) was also tested and showed no significant effect 24 h after addition.