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Structural analysis of the putative regulatory region of the rat gene encoding poly(ADP‐ribose) polymerase
Author(s) -
Potvin Frédéric,
Thibodeau Jacques,
Kirkland James B.,
Dandenault Brigitte,
Duchaine Caroline,
Poirier Guy G.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80457-r
Subject(s) - gene , exon , nucleic acid sequence , polymerase , biology , microbiology and biotechnology , homology (biology) , genetics , sequence analysis , rna polymerase , genomic dna , dna , consensus sequence , nucleotide , peptide sequence , rna
A γ EMBL3 clone containing the first three exons along with part of the 4th exon of the rat poly(ADP‐ribose) polymerase gene was isolated from a genomic DNA library. This clone also contains 6.6 kbp of upstream sequences. Nucleotide sequence analysis of the proximal 5′ 670 nucleotides flanking the major RNA start site of the rat gene does not reveal significant global homology with the same region of the human gene, but a series of short sequences are identical. Among these sequences are found two putative Spl binding sites along with a decanucleotide sequence responsible for the attachment of the transcription factor AP‐2.