Premium
Cloning and sequencing of the 23 kDa mouse photoreceptor cell‐specific protein
Author(s) -
McGinnis James F.,
Stepanik Phillip L.,
Baehr Wolfgang,
Subbaraya Iswari,
Lerious Valentine
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80433-h
Subject(s) - cloning (programming) , microbiology and biotechnology , chemistry , photoreceptor cell , biology , biochemistry , retinal , computer science , programming language
The 23 kDa protein was localized by immunocytochemistry to photoreceptor cells of the mouse retina, and bovine and mouse cDNA clones were isolated and sequenced. The deduced amino acid sequences showed that the mouse 23 kDa protein is 91% identical to the bovine protein, and is the same as S‐modulin, the CAR (cancer‐associated retinopathy) protein and recoverin, the Ca 2+ ‐dependent activator of photoreceptor guanylate cyclase. The amino acid sequence reveals two Ca 2+ binding sites, no internal repeats, 59% homology to the chicken visinin protein and 40% homology to calmodulin while Northern analysis demonstrated a single 1.0 kb mRNA species in bovine and mouse retina.