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Oligo(2′‐ O ‐methyl)ribonucleotides Effective probes for duplex DNA
Author(s) -
Shimizu Miho,
Konishi Aritomo,
Shimada Yasuaki,
Inoue Hideo,
Ohtsuka Eiko
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80428-j
Subject(s) - duplex (building) , chemistry , dna , biophysics , biochemistry , biology
To find novel probes for duplex DNA, we prepared four types of triplexes containing a homopurine‐homopyrimidine 15‐mer duplex DNA, and examined their thermal stabilities ( T m values). The single strands used for triplex formation were a DNA 15‐mer having a defined C‐T mixed sequence, and its sugar‐modified analogs, namely 2′‐fluoro DNA, RNA, and 2′‐ O ‐methyl RNA. The 2′‐ O ‐methyl RNA and the RNA‐containing triplexes were similar in their enhanced stabilities at pH 6.1 and, amongst the four triplexes, the 2′‐ O ‐methyl was the most stable at pH 5.0. Furthermore, an experiment using a 34‐mer duplex DNA suggested that the 2′‐ O ‐methyl RNA‐triplex was destabilized, mostly as a result of the incorporation of a mismatched triplet, as compared to the DNA triplex counterpart. Thus, 2′‐ O ‐methyl RNA can serve as an effective probe for duplex DNA.