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Characterization of the yeast BMH1 gene encoding a putative protein homologous to mammalian protein kinase II activators and protein kinase C inhibitors
Author(s) -
van Heusden G.Paul H.,
Wenzel Thibaut J.,
Lagendijk Ellen L.,
de Steensma H.Y.,
van den Berg Johan A.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80426-h
Subject(s) - protein kinase a , gene , homologous chromosome , yeast , map3k7 , c raf , biology , mitogen activated protein kinase kinase , biochemistry , map2k7 , cyclin dependent kinase 9 , kinase , microbiology and biotechnology , cyclin dependent kinase 2 , chemistry , genetics
We describe the identification and characterization of the BMH1 gene from the yeast Saccharomyces cerevisiae . The gene encodes a putative protein of 292 amino acids which is more than 50% identical with the bovine brain 14‐3‐3 protein and proteins isolated from sheep brain which are strong inhibitors of protein kinase C. Disruption mutants and strains with the BMH1 gene on multicopy plasmids have impaired growth on minimal medium with glucose as carbon source, i.e. a 30–50% increase in generation time. These observations suggest a regulatory function of the bmh1 protein. In contrast to strains with an intact or a disrupted BMH1 gene, strains with the BMH1 gene on multicopy plasmids hardly grew on media with acetate or glycerol as carbon source.