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Nucleotide sequence of a cDNA encoding human tumor necrosis factor β from B lymphoblastoid cell RPMI 1788
Author(s) -
Matsuyama Noriko,
Okawa Noriyuki,
Tsukii Yuji,
Endo Tohru,
Kaji Akira
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80425-g
Subject(s) - complementary dna , microbiology and biotechnology , exon , biology , gene , nucleic acid sequence , asparagine , coding region , peptide sequence , genomic dna , genetics , amino acid
Published sequences of cDNA for human tumor necrosis factor β (TNF‐β) have a discrepancy within the coding region as well as exon 1. To resolve these discrepancies we have re‐isolated TNF‐β cDNA from the human B cell lymphoblastoid cell line, RPMI 1788, and determined its DNA sequence. Results indicate that amino acid 26 is threonine (Thr) instead of asparigine (Asn). In contrast to published sequences, the sequence of the exon 1 region corresponded to the genomic sequence of TNF‐β. From our studies we conclude that the TNF‐β gene of the human B cell lymphoblastoid cell line, RPMI 1788, is homologous with respect to the TNF‐β gene.