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Effective detergent/lipid ratios in the solubilization of phosphatidylcholine vesicles by Triton X‐100
Author(s) -
Partearroyo M.Aránzazu,
Urbaneja M.Angeles,
Goñi Félix M.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80424-f
Subject(s) - vesicle , phosphatidylcholine , phospholipid , pulmonary surfactant , chemistry , micelle , solubilization , chromatography , lipid bilayer , molar ratio , octyl glucoside , triton x 100 , membrane , liposome , bilayer , biophysics , biochemistry , aqueous solution , organic chemistry , biology , catalysis
Effective detergent:lipid ratios (i.e. molar ratios in the mixed aggregates, vesicles or micelles) have been estimated for the solubilization of phosphatidylcholine vesicles by Triton X‐100. Effective molar ratios are given for both the onset and the completion of bilayer solubilization; small unilamellar, large unilamellar and multilamellar vesicles have been used. Effective detergent:lipid ratios are independent of phospholipid concentration, and their use allows a deeper understanding of membrane‐surfactant interactions.