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Primary structure of rat pancreatic lipase mRNA
Author(s) -
Wicker-Planquart Catherine,
Puigserver Antoine
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80403-4
Subject(s) - nucleotide , peptide sequence , amino acid , protein primary structure , messenger rna , nucleic acid sequence , signal peptide , genbank , open reading frame , biochemistry , microbiology and biotechnology , biology , lipase , chemistry , enzyme , gene
The sequence of a rat pancreatic lipase mRNA was determined. The data have been assigned the following accession number, X61925, in the EMBL data library. The total length of the messenger is 1531 nucleotides, plus a poly(A) stretch of about 60 nucleotides. A 72‐nucleotides 5′‐noncoding region is followed by a 1419‐nucleotides open reading frame which encodes a protein of 473 amino acids, including the 17 amino acid signal peptide. The mature enzyme (456 residues) has 6 additional C‐terminal amino acids, as compared with the amino acid sequence of pig (direct amino acid sequence), dog, man and rat isoenzyme from Genbank, M58369 (all deduced from the nucleotide sequence). A higher degree of homology exists between the amino acid sequence of rat mature enzyme with those of dog (88%), pig (75%) and man (75%) than with that of rat isolipase (74%).