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Molecular cloning of a human gene (S31) encoding a novel serotonin receptor mediating inhibition of adenylyl cyclase
Author(s) -
Levy Finn Olav,
Gudermann Thomas,
Birnbaumer Mariel,
Kaumann Alberto J.,
Birnbaumer Lutz
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80379-u
Subject(s) - adenylyl cyclase , g protein coupled receptor , biology , microbiology and biotechnology , 5 ht5a receptor , serotonin , camp dependent pathway , 5 ht receptor , adcy6 , open reading frame , adcy9 , adcy10 , receptor , biochemistry , gene , peptide sequence
We report the molecular cloning of human gene (S31) containing an open reading frame of 1095 nucleotides, which encodes a protein of 365 amino acids. The encoded protein contains seven hydrophobic putative transmembrane domains considered the hallmark of G protein‐coupled receptors. The amino acid sequence shows highest homology to receptors for serotonin (5‐hydroxytryptamine). Expression of this receptor in murine L tk − cells conferred upon these cells the ability to respond to serotonin by inhibition of adenylyl cyclase. No response was observed to isoproterenol, epinephrine, histamine, dopamine or melatonin in the transfected cells. We propose that the human gene S31 encodes a novel serotonin receptor.