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Differential regulation of metalloprotease steady‐state mRNA levels by IL‐1 and FGF in rabbit articular chondrocytes
Author(s) -
Chandrasekhar Srinivasan,
Harvey Anita K.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80378-t
Subject(s) - cycloheximide , collagenase , fibroblast , chondrocyte , interleukin , fibroblast growth factor , autocrine signalling , messenger rna , chemistry , microbial collagenase , microbiology and biotechnology , endocrinology , cytokine , metalloproteinase , biology , medicine , enzyme , protein biosynthesis , immunology , biochemistry , in vitro , receptor , gene
The expression of collagenase and stromelysin is believed to be coordinately regulated. In this report however, we provide evidence that suggests subtle differences may exist in the early events of the induction of these enzymes. Rabbit articular chondrocytes treated with interleukin‐1, either alone or in combination with fibroblast growth factor, accumulated steady‐state mRNA levels for both the enzymes, with the latter treatment more effective in inducing greater levels and within a shorter time. Further, the induction of the enzymes by either protocol was blocked by cycloheximide co‐treatment. Cycloheximide added 1 h post‐stimulation with interleukin‐1 + fibroblast growth factor failed to block stromelysin mRNA expression, but was able to block collagenase steady‐state mRNA levels. Transforming growth factor‐β, another inhibitor of metalloprotease induction, showed no such differential activity. The results suggest that collagenase and stromelysin may have subtle variations in their induction pathways. Our studies further show that the enzyme induction by interleukin‐1 alone or in combination with fibroblast growth factor occurs through different, but related mechanisms.