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Induction of nitric oxide synthase by lipoteichoic acid from Staphylococcus aureus in vascular smooth muscle cells
Author(s) -
Auguet Michel,
Lonchampt Marie-Odile,
Delaflotte Sylvie,
Goulin-Schulz Jocelyne,
Chabrier Pierre Etienne,
Braquet Pierre
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80356-l
Subject(s) - lipoteichoic acid , nitric oxide , nitric oxide synthase , vascular smooth muscle , staphylococcus aureus , contractility , chemistry , intracellular , lipopolysaccharide , biochemistry , atp synthase , methylene blue , biology , microbiology and biotechnology , smooth muscle , bacteria , endocrinology , enzyme , organic chemistry , photocatalysis , catalysis , genetics
Inducible vascular nitric oxide synthase accounts for the contractile impairment observed in ondotoxemia. We provide evidence that lipoteichoic acid (LTA) from Staphylococcus aureus , a micro‐organism without endotoxin, also induces nitric oxide synthase. Our study demonstrates that on endothelium‐free rings of rat aorta. LTA‐like lipopolysaccharide induces a loss of contractility restored by Methylene blue and N G ‐nitro‐ l ‐arginine‐methyl ester (LNAME). Moreover in cultured vascular smooth muscle cells, LTA produces a dose‐dependent increase in intracellular cyclic GMP which is antagonized by LNAME and prevented by dexamethasone.