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Molecular analysis of a new cytoplasmic male sterile genotype in sunflower
Author(s) -
Spassova Mariana,
Christov Michail,
Bohorova Natasha,
Petrov Peter,
Dudov Kalin,
Atanassov Atanas,
Nijkamp H.John J.,
Hille Jaques
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80350-p
Subject(s) - cytoplasmic male sterility , biology , helianthus annuus , sunflower , genotype , restriction fragment length polymorphism , genetics , sterility , software maintainer , locus (genetics) , mitochondrial dna , restriction enzyme , gene , southern blot , helianthus , cleaved amplified polymorphic sequence , hybrid , botany , horticulture
Mitochondrial DNA from 1 fertile and 6 cytoplasmic male sterile (CMS) sunflower genotypes was studied. The CMS genotypes had been obtained either by specific crosses between different Helianthus species or by mutagenesis. CMS‐associated restriction fragment length polymorphisms (RFLPs) were found in the vicinity of the atpA locus, generated by various restriction enzymes. The organization of the mitochondrial genes 26S rRNA , 18S + 5S rRNA and coxII was investigated by Southern blot analysis. These genes have similar structures in fertile and all studied sterile sources. Using the atpA probe, 5 from the 6 investigated CMS genotypes showed identical hybridization patterns to the Petiolaris CMS line, which is used in all commercial sunflower hybrids. Only 1 cytoplasm derived from an open pollination of Helianthus annuus ssp. texanus , known as ANT 1 , contained a unique mithochondrial DNA fragment, which is distinguishable from the fertile and sterile Petiolaris genotypes and from all investigated CMS genotypes. Male fertility restoration and male sterility maintenance of the ANT 1 line are different from the Petiolaris CMS system, which is a confirmation that a novel CMS genotype in sunflower has been identified.