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Cloning and expression of a bovine adenosine A 1 receptor cDNA
Author(s) -
Tucker Amy L.,
Linden Joel,
Robeva Anna S.,
D'Angelo Drew D.,
Lynch Kevin R.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80338-h
Subject(s) - cloning (programming) , complementary dna , microbiology and biotechnology , expression cloning , chemistry , biology , computational biology , biochemistry , gene , computer science , programming language
A bovine brain adenosine A 1 receptor cDNA encoding a 326 amino acid protein has been identified. This cDNA, which encodes a protein >90% identical to analogous rat and dog receptors, was transiently expressed in COS‐1 cells. Recombinant receptors exhibited the features of bovine A 1 receptors that distinguish it from rat and canine receptors, including subnanomolar K i for 1,3‐dipropyl‐8‐cyclopentylxanthine, R ‐phenylisopropyl‐adenosine ( R ‐PIA) and xanthine amino conjugate, and the distinct potency order: R ‐PIA > S ‐PIA > 5′‐ N ‐ethylcarboxamidoadenosine > 2′‐chloroadenosine. The results indicate that the pharmacological differences between A 1 adenosine receptors among species result from only minor differences in receptor structures.