Premium
Intracellular delivery of protein kinase C‐α or ε isoform‐specific antibodies promotes acquisition of a morphologically differentiated phenotype in neuroblastoma cells
Author(s) -
Leli Ubaldo,
Parker Peter J.,
Shea Thomas B.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80334-d
Subject(s) - neuroblastoma , gene isoform , protein kinase c , intracellular , phenotype , microbiology and biotechnology , biology , cellular differentiation , kinase , cell culture , biochemistry , genetics , gene
The protein kinase C (PKC) family participates in a ubiquitous cell signalling system utilizing increased turnover of phosphoinositides. Because down‐regulation of total PKC activity has been implicated in the acquisition of a morphologically differentiated phenotype in SH‐SY5Y neuroblastoma cells, we aimed to identify the specific PKC isoforms in this process. Here we report that intracellular delivery of PKC‐α and ‐ε, but not ‐β |4 ‐γ or ‐δ isoform‐specific antibodies is sufficient to induce acquisition of a morphologically differentiated phenotype in SH‐SY5Y neuroblastoma cells.