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A short form of the tick‐borne encephalitis virus NS3 protein
Author(s) -
Pugachev K.V.,
Nomokonova N.Yu.,
Morozova O.V.,
Pletnev A.G.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80329-f
Subject(s) - ns3 , tick borne encephalitis virus , virology , recombinant dna , monoclonal antibody , virus , biology , encephalitis , antibody , reticulocyte , plasmid , microbiology and biotechnology , gene , messenger rna , biochemistry , immunology , hepatitis c virus
Using monoclonal antibodies to the tick‐borne encephalitis virus (TBE) nonstructural protein NS3 two forms of this protein were revealed in TBE‐infected mammalian cells: a full‐length form (69 kDa) and a short form (49 kDa) which has not been observed before and was called NS3′. Recombinant plasmids were constructed and various fragments of the TBE NS3 gene were expressed in rabbit reticulocyte lysate. By analyzing immune precipitates of 35 S‐labeled translation products, we could monitor and localize internal cleavage of NS3, due to which the NS3′ protein was generated.