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A novel 15 N‐labeling method to selectively observe 15 NH 2 resonances of proteins in 1 H‐detected heteronuclear correlation spectroscopy
Author(s) -
Tate Shin-ichi,
Tate Naoko U.,
Ravera Mark W.,
Jaye Michael,
Inagaki Fuyuhiko
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80322-8
Subject(s) - heteronuclear molecule , heteronuclear single quantum coherence spectroscopy , chemistry , spectroscopy , asparagine , side chain , analytical chemistry (journal) , nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , crystallography , stereochemistry , amino acid , physics , chromatography , biochemistry , organic chemistry , quantum mechanics , polymer
An experimental method to selectively label side‐chain NH 2 groups of glutamine and asparagine in proteins with 15 N is proposed. This selective labeling method enables to observe only 15 NH 2 resonances and thus, to discriminate between 15 NH and 15 NH 2 resonances in a 1 H‐detected heteronuclear correlation spectrum. This method gives results with approximately two times higher sensitivity than those obtained by elaborate pulse sequences such as DEPT‐HSQC and will be useful for studying the molecular interaction involving the side chains of Asn and Gln residues.