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Both the anaerobic pathway and aerobic desaturation are involved in the synthesis of unsaturated fatty acids in Vibrio sp. strain ABE‐1
Author(s) -
Morita Naoki,
Gotoh Masura,
Okajima Nobuhiro,
Okuyama Hidetoshi,
Hayashi auShoichi Higashi Hideyuki,
Murata Norio
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80316-9
Subject(s) - anaerobic exercise , strain (injury) , chemistry , microbiology and biotechnology , vibrio , bacterial strain , biochemistry , bacteria , biology , genetics , physiology , anatomy
Vibrio sp. strain ABE‐1 is a unique marine bacterium in terms of its ability to synthesize Δ9‐ trans ‐hexadecenoic acid and Δ7‐ cis ‐tetradecenoic acid (14:1(7c); Okuyama, H., Sasaki, S., Higashi, S. and Murata, N. (1990) J. Bacteriol. 172, 3515‐3518). The present study, involving labeling with [1‐ 14 C]acetate, demonstrated that 14:1 is synthesized by the anaerobic pathway. When cells of this bacterium were grown in the presence of [1‐ 14 C]myristic acid (14:0), this compound was converted to palmitic (16:0) and hexadecenoic (16:1) acids but not to 14:1, under aerobic conditions. These results suggest that the incorporated 14:0 was elongated to 16:0 and then converted to 16:1 by the aerobic desaturation of 16:0. It appears that the anaerobic pathway and aerobic desaturation are both involved in the synthesis of unsaturated fatty acids during aerobic growth of Vibrio sp. strain ABE‐1.