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Imidazole‐SDS‐Zn reverse staining of proteins in gels containing or not SDS and microsequence of individual unmodified electroblotted proteins
Author(s) -
Ortiz Maria Luisa,
Calero Miguel,
Patron Carlos Fernandez,
Castellanos Lila,
Mendez Enrique
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80309-5
Subject(s) - staining , coomassie brilliant blue , acrylamide , chemistry , chromatography , polyacrylamide gel electrophoresis , gel electrophoresis , electrophoresis , agarose , biochemistry , microbiology and biotechnology , biology , enzyme , organic chemistry , polymer , genetics , copolymer
A reverse staining procedure is described for the detection of proteins in acrylamide and agarose gels with and without SDS. Protein detection occurs a few minutes after electrophoresis. The sensitivity on acrylamide gels is higher than that of Coomassie blue staining either on acrylamide gels or on electrotransferred membranes. Sequencing of protein bands only detected by reverse staining on the gel and not by Coomassie blue is demonstrated.