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Ca 2+ ‐induced Ca 2+ release in insulin‐secreting cells
Author(s) -
Islam Md.Shahidul,
Rorsman Patrik,
Berggren Per-Olof
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80306-2
Subject(s) - insulin , calcium , chemistry , radiochemistry , biophysics , endocrinology , biology , organic chemistry
The sulphydryl reagent thimerosal (50 μM) released Ca 2+ from a non‐mitochondrial intracellular Ca 2+ pool in a dose‐dependent manner in permeabilized insulin‐secreting RINm5F cells. This release was reversed after addition of the reducing agent dithiothreitol, Ca 2+ was released from an Ins(1,4,5)P 3 ‐insensitive pool, since release was observed even after depletion of the Ins(1,4,5)P 3 ‐sensitive pool by a supramaximal dose of Ins(2,4,5)P 3 or thapsigargin. The Ins(1,4,5)P 3 ‐sensitive pool remained essentially unaltered by thimerosal. Thimerosal‐induced Ca 2+ release was potentiated by caffeine. These findings suggest the existence of Ca 2+ ‐induced Ca 2+ release also in insulin‐secreting cells.