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Isolation of factor Xa from chick embryo as the amniotic endoprotease responsible for paramyxovirus activation
Author(s) -
Gotoh Bin,
Yamauchi Fumiko,
Ogasawara Tomohiko,
Nagai Yoshiyuki
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80303-x
Subject(s) - embryo , isolation (microbiology) , chemistry , amniotic fluid , microbiology and biotechnology , virology , biology , biochemistry , genetics , pregnancy , fetus
In chick embryo, certain paramyxoviruses mainly target the chorioallantois and the allantoamnion and show no extensive further spreading in the other organs. This has been explained by the possible presence of an endoprotease activating the viral fusion glycoprotein precursor in the allantoic and the amniotic fluid, and its absence in other places or organs, We previously isolated such an endoprotease from the allantoic fluid and demonstrated its identity with the clotting factor Xa. Exactly the same endoprotease by all the criteria including the N‐terminal amino acid sequence was now isolated from the amniotic fluid. Thus, the factor Xa seems to be a major host determinant of the viral tropism in chick embryo.