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Cellobiose oxidase of Phanerochaete chrysosporium enhances crystalline cellulose degradation by cellulases
Author(s) -
Bao Wenjun,
Renganathan V.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80289-s
Subject(s) - phanerochaete , cellulase , cellobiose , chrysosporium , cellulose , cellobiose dehydrogenase , chemistry , degradation (telecommunications) , biochemistry , food science , enzyme , telecommunications , computer science
The effect of Phanerochuete chrysosporium cellobiose oxidase (CBO) on microcrystalline cellulose hydrolysis by Trichoderma cellulases was determined. Addition of 10 μg ml −1 CBO to a reaction mixture containing T. viride cellulase increased glucose and cellobiose production by 10% and 48%, respectively. Cellulose weight loss was also enhanced by 19%. Al higher concentrations (20–80 μg ml −1 ). CBO decreased glucose and cellobiose production. Cellulose weight loss at 60 μ ml −1 CBO was 76% compared to control cellulase reactions. This decrease appears to be due to inactivation of cellulase by H 2 O 2 produced via CBO reaction, because addition of catalase enhances sugar production and cellulose weight loss. These findings suggest that at low, perhaps physiologically relevant concentrations, CBO enhances crystalline cellulose degradation by cellulases.