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Kinetic analysis of translocation into isolated chloroplasts of the purified ferredoxin precursor
Author(s) -
Pilon Marinus,
Weisbeek Peter J.,
de Kruijff Ben
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80286-p
Subject(s) - ferredoxin , chloroplast , cytosol , biochemistry , chemistry , biology , enzyme , gene
Time courses of the import into isolated chloroplasts of the purified ferredoxin precursor were measured at different protein concentrations. Analysis of the initial import rates indicates the presence of one saturable import system with an apparent K m value of approximately 100 nM and a V max corresponding to the uptake of approximately 2.5·10 4 precursor proteins per minute per chloroplast. We conclude that the in vitro observed activity of the chloroplast protein import machinery, functioning independently from cytosolic factors. would be enough to allow chloroplast developmental at physiological rate.