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C‐CAM (Cell‐CAM 105) is a calmodulin binding protein
Author(s) -
Blikstad Ingrid B.,
Wikström Tarja,
Aurivillius Magnus,
Öbrink Björn
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80276-m
Subject(s) - calmodulin , transmembrane protein , western blot , gene isoform , cytoplasm , chemistry , binding protein , microbiology and biotechnology , cell adhesion molecule , biochemistry , cell adhesion , cell , biology , enzyme , receptor , gene
C‐CAM (Cell‐CAM 105) is a transmembrane cell adhesion molecule belonging to the immunoglobulin superfamily. It mediates intercellular adhesion of rat hepatocytes and occurs in various isoforms in several epithelia, vessel endothelia and leukocytes. We now report that purified liver C‐CAM interacts specifically with calmodulin. Binding was observed both when 125 I‐labeled C‐CAM was used in a dot‐blot assay and when 125 I‐labeled calmodulin was used in a gel overlay assay. Experiments with protease‐generated peptides indicated that calmodulin bound to the cytoplasmic domain of C‐CAM. Analyses of whole liver membranes demonstrated that C‐CAM is one of five major proteins that bind calimodulin in a calcium‐dependent manner.