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Production of a unique multi‐lamella structure in the nuclei of yeast expressing Drosophila copia gag precursor
Author(s) -
Yoshioka Katsuji,
Fujita Atsushi,
Kondo Shunzo,
Miyake Tadashi,
Sakaki Yoshiyuki,
Shiba Tadayoshi
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80270-q
Subject(s) - retrotransposon , yeast , drosophila melanogaster , microbiology and biotechnology , biology , reverse transcriptase , cytoplasm , lamella (surface anatomy) , rna , chemistry , gene , genetics , genome , transposable element , anatomy
Drosophila retrotransposon copia produces virus‐like particles (VLPs) in the nuclei of cultured Drosophila cells. The VLPs contain copia RNA and reverse transcriptase activity, and thus, play a major role in copia replication. Here we have expressed the copia gag polyprotein precursor in yeast. The precursor, which includes copia protease itself, showed correct autoprocessing to produce a unique multi‐lamella structure in the nuclei of the yeast cells. This expression system should be useful for the analysis of nuclear localization ot'the major copia VLP protein, and Furthermore, would provide important information concerning the mechanism of copia VLPs formation.