Premium
Inserting foreign peptides into the major coat protein of bacteriophage M13
Author(s) -
Ilyichev A.A.,
Minenkova O.O.,
Kishchenko G.P.,
Tat'kov S.I.,
Karpishev N.N.,
Eroshkin A.M.,
Ofitzerov V.I.,
Akimenko Z.A.,
Petrenko V.A.,
Sandakhchiev L.S.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80267-k
Subject(s) - coat protein , bacteriophage , dna , infectivity , gene , chemistry , biology , microbiology and biotechnology , biochemistry , virus , virology , escherichia coli , rna
Foreign DNA fragments were inserted into filamentous phage gene VIII to create hybrid B‐proteins with foreign sequences in the amino terminus. The hybrid proteins are incorporated into the virions which retain viability and infectivity. Virions with hybrid B‐proteins have the same contour length and the same number of B‐protein molecules as virions with natural B‐proteins. It was shown that for one of hybrid B‐proteins the position of the processing site had changed.