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Effects of pressure on glucose transport in human erythrocytes
Author(s) -
Thorne S.D.,
Hall A.C.,
Lowe A.G.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80261-e
Subject(s) - hydrostatic pressure , chemistry , glucose transporter , transporter , cytochalasin b , biophysics , biochemistry , cell , biology , thermodynamics , endocrinology , physics , gene , insulin
The operation of the human red cell glucose transporter has been studied at normal and high hydrostatic pressure to identify the step(s) which involve a volume change. Pressure inhibited zero‐ trans and equilibrium exchange influx to similar extents, by decreasing the V max but not significantly changing the K m . The B max and K d of specific [ 3 H]cytochalasin B binding were unaffected by pressure indicating no change to the number or affinity of functional transporters at pressure. Passive glucose transport was inhibited by pressure in a manner consistent with permeation across the lipid bilayer. These data indicate that there is a major change in volume during the translocation step of the glucose transporter which is rate‐limiting for transport.