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Metabotropic responses to acetylcholine and serotonin of Xenopus oocytes injected with rat brain mRNA are transduced by different G‐protein subtypes
Author(s) -
Kaneko Shuji,
Takahashi Hideki,
Satoh Masamichi
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80242-9
Subject(s) - xenopus , muscarinic acetylcholine receptor , phospholipase c , biology , acetylcholine , messenger rna , microbiology and biotechnology , second messenger system , oocyte , receptor , medicine , endocrinology , signal transduction , biochemistry , gene , embryo
To assign the GTP‐binding protein (G‐protein) subtype involved in the signal transduction from exogenous receptors to phospholipase C in the Xenopus oocyte translation system, antisense DNA complementary to rat G‐protein α‐subunit MRNA was designed and injected together with rat brain poly(A) + RNA. Current response of mRNA‐injected oocytes to acetylcholine (ACh) was suppressed dose‐dependently by a co‐injection of G il α‐antisense DNA, but response of the same oocytes to serotonin (5‐HT) was not inhibited. In the oocytes co‐injected with G o α‐antisense DNA, the 5‐HT response was more effectively suppressed than the ACh response. These results suggest that G o α but not G il α intermediates brain 5‐HT IC receptor function, and in contrast, muscarinic receptors derived from rat brain utilize G il α rather than G o α to activate phospholipase C.