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Amino acid sequence of the unique protamine from yellow perch
Author(s) -
Chao Heman,
Davies Peter L.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80239-d
Subject(s) - protamine , perch , peptide sequence , amino acid , chemistry , sequence (biology) , biochemistry , biology , fish <actinopterygii> , fishery , gene , heparin
By the criteria of gel electrophoresis, ion‐exchange chromatography, and reverse‐phase HPLC, yellow perch prolamine behaves as a single component. This observation was confirmed by automated Edman degradation which gave a single unambiguous amino acid sequence PRRRRHAARPVTRRSSRVHRRRRAVRRRR. Yellow perch prolamine has 34 amino acids, including 21 arginines. It has two histidines, neither of which interrupts an arginine tract. It is unusual among fish protamines in not having a serine or threonine N‐terminal to the second arginine tract, and is unique in not being a mixture of components.