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Phorbol ester‐induced secretion of human hepatocyte growth factor by human skin fibroblasts and its inhibition by dexamethasone
Author(s) -
Gohda Eiichi,
Kataoka Hirotoshi,
Tsubouchi Hirohito,
Daikilara Yasushi,
Yamamoto Itaru
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80220-b
Subject(s) - phorbol ester , secretion , hepatocyte growth factor , dexamethasone , chemistry , hepatocyte , growth factor , endocrinology , medicine , microbiology and biotechnology , biology , protein kinase c , biochemistry , in vitro , signal transduction , receptor
Human skin fibroblasts secreted a certain amount of human hepatocyte growth factor (hHGF), as determined by an enzyme‐linked immunosorbent assay for hHGF. This hHGF secretion was remarkably stimulated by protein kinase C (PKC)‐activating phorbol esters, which was inhibited by the simultaneous addition of dexamethasone. Pretreatment with phorbol 12‐myristate 13‐acetate (PMA) caused a down‐regulation in hHGF secretion. hHGF secreted by the PMA‐treated cells showed a potent hepatocyle growth‐promoting activity which was neutralized by an anti‐hHGF antiserum. These results indicate both that PMA‐treated human skin fibroblasts produce biologically active hHGF and the possible involvement of PKC activation in this process.