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Stimulation of glucose transport in L6 muscle cells by long‐term intermittent stretch—relaxation
Author(s) -
Mitsumoto Yasuhide,
Downey Gregory P.,
Klip Amira
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80217-5
Subject(s) - glucose transporter , glut4 , medicine , stimulation , myocyte , endocrinology , cycloheximide , glucose uptake , myogenesis , skeletal muscle , glut1 , chemistry , muscle hypertrophy , biology , insulin , biochemistry , protein biosynthesis
Skeletal muscle stretch increases resting metabolism and causes hypertrophy. We have examined the effect of mechanical stretch in vitro on glucose transport activity and transporter contents in L6 muscle cells. Long‐term (24–48 h) stretch—relaxation (25% maximal elongation at 30 cycles per min) of cell monolayers significantly increased glucose uptake by 1.6‐ to 2‐fold in myotubes but not in myoblasts. The presence of serum was required for the stretch—relaxation induced increase in glucose uptake. Cycloheximide inhibited the mechanical stimulation of glucose uptake, and the latter response was not additive to the stimulatory effect of long‐term exposure to insulin. GLUT1 and GLUT4 glucose transporter contents were not changed in total cell membranes from mechanically stimulated cells relative to controls. These results indicate that mechanical stimulation through passive stretch may be an important regulation of nutrient uptake in fetal myotubes independent of innervation.