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Rainbow trout cytochrome P ‐450 c17 (17α‐hydroxylase/17,20‐lyase) cDNA cloning, enzymatic properties and temporal pattern of ovarian P ‐450 c17 mRNA expression during oogenesis
Author(s) -
Sakai Noriyoshi,
Tanaka Minoru,
Adachi Shinji,
Miller Walter L.,
Nagahama Yoshitaka
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80210-8
Subject(s) - complementary dna , rainbow trout , biology , microbiology and biotechnology , trout , open reading frame , cdna library , peptide sequence , biochemistry , gene , fishery , fish <actinopterygii>
A cDNA clone encoding cytochrome P ‐450 c17 (17α‐hydroxylase/17,20‐lyase) was isolated from a rainbow trout ovarian follicle cDNA library. The cDNA contained an open reading frame of 1,542 nucleotides encoding a protein of 514 amino acid residues. The amino acid sequence of trout P ‐450 c17 shows a much greater homology with chicken P ‐450 c17 than with that of human, bovine and rat. The trout P ‐450 c17 expressed in non‐steroidogenic mammalian COS‐1 cells showed both 17α‐hydroxylase and 17,20‐lyase activities. The cDNA only hybridized to a single species or mRNA (2.4 kb) isolated from rainbow trout ovaries; the 2.4 kb transcripts were abundant in trout ovaries during the later stages of oogenesis.