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Heat‐shock responsive elements in the induction of the multidrug resistance gene ( MDR1 )
Author(s) -
Kioka Noriyuki,
Yamano Yoshiaki,
Komano Tohru,
Ueda Kazumitsu
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80205-u
Subject(s) - sodium arsenite , multiple drug resistance , arsenite , gene , gene expression , biology , microbiology and biotechnology , heat shock protein , promoter , hsp70 , cell culture , chemistry , genetics , drug resistance , arsenic , organic chemistry
The MDR1 gene, considered to be involved in multidrug resistance of cancer cells, is expressed in liver, kidney, small intestine and the blood‐brain barrier. We investigated MDR1 gene expression in the well‐differentiated hepatoma cell line HepG2 after exposure to several stresses and found that sodium arsenite treatment increased MDR1 gene expression 2.6‐fold. Deletion analysis of the MDR1 promoter indicated that the transcriptional activation after exposure to arsenite depends on a 60‐bp region containing two heat‐shock responsive elements.