Premium
Formation of free sphingosine and ceramide from exogenous ganglioside GM1 by cerebellar granule cells in culture
Author(s) -
Riboni Laura,
Bassi Rosaria,
Sonnino Sandro,
Tettamanti Guido
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80193-k
Subject(s) - ceramide , sphingosine , ganglioside , lipid signaling , sphingolipid , granule (geology) , incubation , intracellular , biochemistry , chemistry , microbiology and biotechnology , biology , apoptosis , enzyme , receptor , paleontology
Cerebellar granule cells differentiated in culture were incubated with ganglioside [ 3 H‐Sph]GM1 in order to have it inserted into the plasma membrane and metabolized. Among the formed metabolites radioactive sphingosine and ceramide were identified. [ 3 H]Ceramide started to be measurable after 10 min of incubation (pulse), and [ 3 H]sphingosine after 15 min. Their concentrations increased with pulse time, and after a 1‐hour pulse, with chase time. After a 1‐hour pulse with 2 × 10 −6 M [ 3 H‐Sph]GM1 followed by a 4‐hour chase, the amount of [ 3 H]sphingosine and [ 3 H]ceramide formed were 0.04 and 0.4 pmol/10 6 cells, respectively. Particularly the ability to produce sphingosine was higher in differentiated than in undifferentiated cells. It is concluded that ganglioside turnover contributes to the maintenance of the intracellular levels of free sphingosine and ceramide.