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Messenger RNA sorting in enterocytes Co‐localization with encoded proteins
Author(s) -
Rings Edmond H.H.M.,
Büller Hans A.,
de Boer Piet A.J.,
Grand Richard J.,
Montgomery Robert K.,
Lamers Wouter H.,
Charles Robert,
Moorman Antoon F.M.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80192-j
Subject(s) - endoplasmic reticulum , messenger rna , lactase , enterocyte , biology , cytosol , microbiology and biotechnology , cytoplasm , translation (biology) , biochemistry , ribosome , rna , enzyme , gene , small intestine
This study describes the intracellular compartmentalization of three different mRNAs in the polarized rat fetal enterocyte. They encode proteins that are known to be localized within different regions of the epithelial cell namely (i) the apical, membrane‐bound glycoprotein, lactase‐phlorizin hydrolase (lactase), (ii) the mitochondrially localized enzyme, carbamoylphosphate synthetase (CPS), and (iii) the cytoplasmically localized enzyme, phospho enol pyruvate carboxykinase (PEPCK). These mRNAs are found in close proximity to their respective protein products, i.e. the apical membrane, mitochondria and cytoplasm, respectively. The significance or these observations is twofold: (i) they indicate that mRNAs are sorted into specific domains of the cytosol of intestinal epithelial cells: and (ii) they imply the presence or two distinct pathways of mRNA targeting one that allows transport of mRNAs that are translated on ribosomes associated with the rough endoplasmic reticulum (lactase mRNA), and the other that allows sorting of mRNAs that are translated on free polysames (CPS and PEPCK mRNA).