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Peroxisomal protein import In vivo evidence for a novel ranslocation competent compartment
Author(s) -
Heinemann Petra,
Just Wilhelm W.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80191-i
Subject(s) - peroxisome , organelle , microbody , differential centrifugation , density gradient , compartment (ship) , biochemistry , vesicle , biology , buoyant density , biogenesis , in vivo , microbiology and biotechnology , chemistry , membrane , receptor , gene , dna , oceanography , physics , quantum mechanics , geology
In homogenates of isolated hepatocytes separated by Nycodenz density gradient centrifugation, two peroxisomal populations are identified that differ in buoyant density. Organelles present in a high density fraction (1.22–1.23 g/cm 3 ) represent mature peroxisomes. Vesicles of intermediate density (1.16–1.17 gm̧ 3 ) represent mature peroxisomes. Vesicles of intermediate density (1.16–1.17 g/cm 3 ) are present in much lower concentration and seem to play a particular role in the import and distribution or newly synthesized peroxisomal proteins. In a typical pulse‐chase experiment with a 7.5 min pulse, peroxisomal acyl‐CoA oxidase is first imported into the peroxisomal fraction of intermediate density. After a chase of up to 30 min, the enzyme is found in mature peroxisomes.