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Evidence for multiple genes coding for the isozymes of hexokinase in the highly glycolytic AS‐30D rat hepatoma
Author(s) -
Wigley W.Christian,
Nakashima Richard A.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80185-j
Subject(s) - hexokinase , isozyme , glycolysis , biochemistry , gene , chemistry , biology , enzyme
We have compared Southern blots of rat hepatoma DNA probed with Types I, II and III hexokinase cDNAs isolated from normal rat tissues. Hybridization patterns show several fragments recognized by both the Type I and II clones while no resemblance is observed between the Type III probe and the other two isozymes. It therefore appears that the Type I‐like and Type‐II‐like hepatoma isozymes are coded for by similar yet separate genes, while a dissimilar third gene codes for the Type III‐like isozyme. In addition, a loss of heterozygosity was detected at the Type III locus in the AS‐30D hepatoma when compared to normal tissues. As only the Type II‐like isozyme is highly expressed in highly glycolytic tumors. these data have implications for differential gene regulation between the turmor isozymes.