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Improved band shift assay for the simultaneous analysis of protein—DNA interactions and enzymatic functions of DNA polymerases
Author(s) -
Strick Reiner,
Knopf Charles W.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80182-g
Subject(s) - dna polymerase , dna polymerase ii , polymerase , dna clamp , primase , microbiology and biotechnology , dna polymerase i , dna polymerase mu , dna , dna replication , biology , biochemistry , chemistry , circular bacterial chromosome , reverse transcriptase , polymerase chain reaction , gene
A simple method to assay the major properties of DNA polymerases such as template binding, polymerase and exonuclease activities in one step is exemplified with the DNA polymerases of E. coli , bacteriophage T4 and herpes simplex virus. Combining the advantages of the band‐shift assay with the resolving power of polyacrylamide gradient gel electrophoresis, the procedure is particularly useful for a rapid functional analysis of mutant polymerases as well as inhibitors of DNA replication.