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Cloning and sequencing of cDNA encoding the putative insect lasma membrane V‐ATPase subunit A
Author(s) -
Gräf Ralph,
Novak Frans J.S.,
Harvey William R.,
Wieczorek Helmut
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80177-i
Subject(s) - complementary dna , manduca sexta , biology , protein subunit , cdna library , peptide sequence , microbiology and biotechnology , midgut , protein primary structure , biochemistry , cloning (programming) , molecular cloning , gene , insect , botany , programming language , larva , computer science
For the first time a cDNA encoding subunit A of an invertebrate V‐ATPase has been sequenced. The cDNA library was prepared from larval midgut of the tobacco hornworm. Manduca sexta , and screened with monoclonal antibodies to the midgut plasma membrane subunit A. From the cDNA sequence the insect subunit A is predicted to consist of 617 amino acids with a relative molecular mass of 68.162. The predicted primary structure is similar to that of the published eukaryotic subunit A proteins ( Bos , Daucus , Saccharomyces and Neurospora ); it most closely resembles the bovine amino acid sequences with which it has an 83% sequence identity