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Probable occurrence of toxin‐susceptible G proteins in the nematode Caenorhabditis elegans
Author(s) -
Arata Yoichiro,
Tada Shusuke,
Ui Michio
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80167-f
Subject(s) - caenorhabditis elegans , toxin , nematode , biology , microbiology and biotechnology , chemistry , genetics , gene , ecology
Pertussis toxins, islet‐activating protein (IAP), and cholera toxin ADP‐ribosylated 40 kDa and 45 kDa proteins in membrane preparations from Caenorhabditis elegans . Proteins with the same molecular weights were recognized in the same membranes by an antibody that had been raised against a peptic common to α‐subunits of mammalian αβγ‐heterotrimeric G proteins. The antibody produced immunoprecipitation with the 40 kDa protein 32 P‐labeled by IAP, A 35 kDa protein immunochemically indistinguishable from β‐component of mammalian G proteins was also found in C. elegans membranes. The membranes displayed adenylate cyclase activity which was highly sensitive to forskolin and GPT analogues, whose action was antagonized by GDPβS. Receptor‐coupled regulation of adenylate cyclase thus appears to be mediated by mammalian‐type G proteins in C. elegans as well.