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The sodium ion pumping oxaloacetate decarboxylase of Klebsiella pneumoniae Metal ion content, inhibitors and proteolytic degradation studies
Author(s) -
Dimroth Peter,
Thomer Anna
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80165-d
Subject(s) - klebsiella pneumoniae , chemistry , sodium , biochemistry , degradation (telecommunications) , metal , metal ions in aqueous solution , microbiology and biotechnology , ion , inorganic chemistry , nuclear chemistry , biology , organic chemistry , escherichia coli , telecommunications , computer science , gene
Oxaloacetate decarboxylase of Klebsiella pneumoniae was shown to contain between 0.6 and 1.0 mol zinc per mol enzyme in different preparations. The decarboxylase activity was completely abolished after 15 min incubation with 1 mM Hg(NO 3 ) 2 in phosphate buffer, while the activity decreased only 20% if the incubation was performed in MES/Trix buffer. Treatment of the isolated subunits with Hg(NO 3 ) 2 indicated that the binding site for Hg 2+ ions is on the α subunit. Other inhibitors of the decarboxylase are KSCN and diethylstilbestrol. Inactivation of the enzyme with 2% 1‐butanol was significantly reduced by 100 mM NaCl. Sodium ions also protected the isolated β+γ subunits from a digestion with trypsin.