Premium
Galanin reduces PDBu‐induced protein phosphorylation in rat ventral hippocampus
Author(s) -
La Porta Caterina,
Bianchi Roberto,
Sozzani Silvano,
Bartfai Tamas,
Consolo Silvana
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)80161-9
Subject(s) - phosphorylation , protein kinase c , galanin , medicine , antagonist , endocrinology , hippocampal formation , protein kinase a , protein phosphorylation , biology , stimulation , basal (medicine) , chemistry , microbiology and biotechnology , neuropeptide , receptor , insulin
The effect of galanin(GAL) on basal and phorbol‐12,13‐dibutyrate (PDBu) induced protein phosphorylation in rat ventral hippocampal miniprisms was investigated. GAL (0.5, 1 and 2μM) inhibited PDBu stimulation in a concentration‐dependent manner without altering basal protein phosphorylation. This inhibitory effect was prevented by the GAL antagonist galantide. GAL did not affect either the activity of protein kinase C (PKC) from rat brain or basal phosphorylation in ventral hippocampal hippogenates, suggesting that it did not directly modulate PKC activity, Depolarization of miniprisms from ventral hippocampl by 18 mM K + prevented the effect of GAL on PDBu‐induced phosphorylation. The results indicate that GAL indirectly regulates neuronal protein phosphorylation by a GAL receptor‐mediated action.